Detection of Oncogenic HPV in Saliva of HIV Infected Patients

1Career Dental College And Hospital, Lucknow, Uttar Pradesh, India, 2Ragas Dental College & Hospital, Chennai, Tamil Nadu, India, 3Ragas Dental College & Hospital, Chennai, India, 4YRG CARE, Chennai, Chennai, India

Objectives: To detect the presence of Oncogenic Human Papilloma Virus in the saliva of HIV seropositive patients in Chennai, South India.

Method: The study was designed to detect the E6, E7 gene of oncogenic HPVs (type 16, 18, 31, 33, 35, 45, 52b and58) by AmpliGeNei TM HPV detection kit in saliva by polymerase chain reaction (PCR). Group 1: Individuals who are HIV seropositive and not on HAART therapy (n=15). Group 2: Individuals who are HIV seropositive and on HAART therapy (n=15). Group 3: Individuals who are HIV seronegative (n=15)

Results: We demonstrated amplification of the positive control and house keeping gene, α-globin. We were unable to detect oncogenic HPV in any of the group1, 2 and 3 samples.

Conclusion: In this cohort we could not detect oncogenic HPVs in any of the groups. Future studies should screen a larger number of patients on HAART therapy for an extended period of time.