HSV-1 IgG and DNA Screening Test in
Sera and Saliva of HIV Patients

Mega Rafika1, Agnes Rengga Indrati2, Irna Sufiawati3

Oral Medicine Residency Program, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia

Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia

Oral Medicine, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia



Objectives: To study the contribution of routine screening of HSV-1 infection using  serum and saliva of HIV patients.


Methods: Cross-sectional analysis samples of was carried out on 58 samples consisting of 29 sera and 29 saliva collected from 29 HIV patients in Dr. Hasan Sadikin General Hospital, Bandung, West Java, Indonesia. HSV-1 IgG antibodies were examined by enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassay (CLIA), while HSV-1 DNA was examined by polymerase chain reaction (PCR).


Results: Using ELISA, HSV-1 IgG antibodies were found high in sera of 23 (79,3%) HIV patients (mean 53,0±12,2 IDU), and 24 (82,8%) (mean 36,8±18,7 IDU) using CLIA. HSV- 1 IgG antibodies were not detected in saliva. HSV-1 DNA was not detected in sera, but we found positive viral DNA in 1 (3.4%) out of the 29 of saliva samples.


Conclusions: HSV-1 IgG antibodies testing in sera showed a high prevalence using  both ELISA and CLIA testing, but antibodies were not detected in saliva - this may be due to its low concentration. HSV-1 DNA test was found in saliva which may demonstrate that an active infection occurred locally.